phycoerythrin pe lag 3  (R&D Systems)

Journal: Reproductive Sciences

Article Title: Circulating Innate Lymphoid Cells Exhibit Distinctive Distribution During Normal Pregnancy

doi: 10.1007/s43032-021-00834-6

Figure Lengend Snippet: The estrogen and progesterone receptors in circulating ILC2s change differently in non-pregnant and pregnant women of different trimesters. a–d Flow cytometric analysis for PR and ERα in peripheral ILC2s of non-pregnant, early-pregnant, and late-pregnant women. a Representative flow cytometry plots are shown in which numbers indicate the frequency of flow cytometric events. b Comparison of and PR + ILC2 and ERα + ILC2 proportion in peripheral blood of non-pregnant women (black circles), early-pregnant women (blue squares), and late-pregnant women (red triangles). c Representative flow cytometry histograms of intracellular PR and ERα in circulating ILC2s from isotype control (grey area), non-pregnant women (black lines), early-pregnant women (blue lines), and late-pregnant women (red lines). d Quantification of PR and ERα mean fluorescence intensity (MFI) of circulating ILC2s. Data are shown as means ± SEMs and were analyzed by one-way ANOVA. * P < 0.05 and *** P < 0.001. ns , not significant

Article Snippet: For measurement of intranuclear estrogen and progesterone receptors, PBMCs were isolated directly ex vivo, firstly stained with antibodies to surface antigens, fixed and permeabilized using Foxp3/Transcription Factor Staining Buffer Set (eBioscience, San Diego, USA) according to the manufacturer’s instructions, and secondly stained with phycoerythrin (PE)-conjugated anti-ERα (ab209288, Abcam, Cambridge, UK) and Alexa Fluor 647-conjugated anti-progesterone receptor (D8Q2J, Cell Signaling Technology, Danvers, USA).

Techniques: Flow Cytometry, Fluorescence